| PubMedID | 23524392 | Journal | Nat Methods, 2013 May;10(5);407-9, |
|---|---|---|---|
| Title | A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. | ||
| Author | Shaner NC, Lambert GG, ..., Davidson MW, Wang J | ||
北海道大学電子科学研究所 光細胞生理研究分野 根本知己 2014/04/11
オワンクラゲ由来のGFPそのものについては蛍光波長のバライエティ等の点において、多くの遺伝子工学的な改変がし尽くされており、もう現状で十分という風潮も存在する。しかし、他の生物種由来の蛍光タンパク質を用いて、より量子収率が高い、発現が容易等の基本的な性質の向上や、計画班員の宮脇先生らのUnaGは言うまでもなく、機能的蛍光タンパク質の探索という潮流も広がっている。この紹介する論文では、ナメクジウオ由来の蛍光タンパク質から単量体で高強度、狭スペクトルのmNeoGreenを作成した。本論文の（個人的に）興味深い点は、（１）立体構造予測やドッキングシミュレーションを用いることでモノマー化（のための変異）を実施していること、（２）超解像イメージングに利用可能であることが強調されていること、である。今後、数理科学、データベースを得意とする共同研究は、多様なライフサイエンスの諸分野で広がっていくことを想像させる。
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| 178 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 179 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 180 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 181 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 182 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 183 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 184 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 185 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 186 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 187 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 188 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 189 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 190 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 191 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 192 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 193 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 194 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 195 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 196 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 197 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 198 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 199 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 200 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 201 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 202 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 203 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 204 | 1 1 -1 OR 2+773-773-1=0+0+0+1 -- | Mr. | 2025/04/20 |
| 1 | |||
| 205 | 1 1 -1 OR 3+773-773-1=0+0+0+1 -- | Mr. | 2025/04/20 |
| 1 | |||
| 206 | 1 1 -1 OR 3*2<(0+5+773-773) -- | Mr. | 2025/04/20 |
| 1 | |||
| 207 | 1 1 -1 OR 3*2>(0+5+773-773) -- | Mr. | 2025/04/20 |
| 1 | |||
| 208 | 1 1 -1 OR 2+413-413-1=0+0+0+1 | Mr. | 2025/04/20 |
| 1 | |||
| 209 | 1 1 -1 OR 3+413-413-1=0+0+0+1 | Mr. | 2025/04/20 |
| 1 | |||
| 210 | 1 1 -1 OR 3*2<(0+5+413-413) | Mr. | 2025/04/20 |
| 1 | |||
| 211 | 1 1 -1 OR 3*2>(0+5+413-413) | Mr. | 2025/04/20 |
| 1 | |||
| 212 | 1 1 -1' OR 2+483-483-1=0+0+0+1 -- | Mr. | 2025/04/20 |
| 1 | |||
| 213 | 1 1 -1' OR 3+483-483-1=0+0+0+1 -- | Mr. | 2025/04/20 |
| 1 | |||
| 214 | 1 1 -1' OR 3*2<(0+5+483-483) -- | Mr. | 2025/04/20 |
| 1 | |||
| 215 | 1 1 -1' OR 3*2>(0+5+483-483) -- | Mr. | 2025/04/20 |
| 1 | |||
| 216 | 1 1 -1' OR 2+847-847-1=0+0+0+1 or 'oKiHk3wf'=' | Mr. | 2025/04/20 |
| 1 | |||
| 217 | 1 1 -1' OR 3+847-847-1=0+0+0+1 or 'oKiHk3wf'=' | Mr. | 2025/04/20 |
| 1 | |||
| 218 | 1 1 -1' OR 3*2<(0+5+847-847) or 'oKiHk3wf'=' | Mr. | 2025/04/20 |
| 1 | |||
| 219 | 1 1 -1' OR 3*2>(0+5+847-847) or 'oKiHk3wf'=' | Mr. | 2025/04/20 |
| 1 | |||
| 220 | 1 1 -1" OR 2+451-451-1=0+0+0+1 -- | Mr. | 2025/04/20 |
| 1 | |||
| 221 | 1 1 -1" OR 3+451-451-1=0+0+0+1 -- | Mr. | 2025/04/20 |
| 1 | |||
| 222 | 1 1 -1" OR 3*2<(0+5+451-451) -- | Mr. | 2025/04/20 |
| 1 | |||
| 223 | 1 1 -1" OR 3*2>(0+5+451-451) -- | Mr. | 2025/04/20 |
| 1 | |||
| 224 | 1 1 if(now()=sysdate(),sleep(15),0) | Mr. | 2025/04/20 |
| 1 | |||
| 225 | 1 1 lxbfYeaa0'XOR(if(now()=sysdate(),sleep(15),0))XOR'Z | Mr. | 2025/04/20 |
| 1 | |||
| 226 | 1 1 lxbfYeaa0"XOR(if(now()=sysdate(),sleep(15),0))XOR"Z | Mr. | 2025/04/20 |
| 1 | |||
| 227 | 1 1 (select(0)from(select(sleep(15)))v)/*'+(select(0)from(select(sleep(15)))v)+'"+(select(0)from(select(sleep(15)))v)+"*/ | Mr. | 2025/04/20 |
| 1 | |||
| 228 | 1 1 lxbfYeaa-1 waitfor delay '0:0:15' -- | Mr. | 2025/04/20 |
| 1 | |||
| 229 | 1 1 lxbfYeaa14c3wsUF'; waitfor delay '0:0:15' -- | Mr. | 2025/04/20 |
| 1 | |||
| 230 | 1 1 lxbfYeaaiKKz2qr4' OR 330=(SELECT 330 FROM PG_SLEEP(15))-- | Mr. | 2025/04/20 |
| 1 | |||
| 231 | 1 1 lxbfYeaa3oTJ8tpu') OR 103=(SELECT 103 FROM PG_SLEEP(15))-- | Mr. | 2025/04/20 |
| 1 | |||
| 232 | 1 1 lxbfYeaakggh7LVQ')) OR 171=(SELECT 171 FROM PG_SLEEP(15))-- | Mr. | 2025/04/20 |
| 1 | |||
| 233 | 1 1 lxbfYeaa'||DBMS_PIPE.RECEIVE_MESSAGE(CHR(98)||CHR(98)||CHR(98),15)||' | Mr. | 2025/04/20 |
| 1 | |||
| 234 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 235 | 1 1 lxbfYeaa'" | Mr. | 2025/04/20 |
| 1 | |||
| 236 | 1 1 lxbfYeaa是摺%2527%2522\'\" | Mr. | 2025/04/20 |
| 1 | |||
| 237 | 1 1 @@iZHUw | Mr. | 2025/04/20 |
| 1 | |||
| 238 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 239 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 240 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 241 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 242 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 243 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 244 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 245 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 246 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 247 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 248 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 249 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 250 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 251 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 252 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 253 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 254 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 255 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 256 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 257 | 1 1 lxbfYeaa | -1 OR 2+685-685-1=0+0+0+1 -- | 2025/04/20 |
| 1 | |||
| 258 | 1 1 lxbfYeaa | -1 OR 3+685-685-1=0+0+0+1 -- | 2025/04/20 |
| 1 | |||
| 259 | 1 1 lxbfYeaa | -1 OR 3*2<(0+5+685-685) -- | 2025/04/20 |
| 1 | |||
| 260 | 1 1 lxbfYeaa | -1 OR 3*2>(0+5+685-685) -- | 2025/04/20 |
| 1 | |||
| 261 | 1 1 lxbfYeaa | -1 OR 2+810-810-1=0+0+0+1 | 2025/04/20 |
| 1 | |||
| 262 | 1 1 lxbfYeaa | -1 OR 3+810-810-1=0+0+0+1 | 2025/04/20 |
| 1 | |||
| 263 | 1 1 lxbfYeaa | -1 OR 3*2<(0+5+810-810) | 2025/04/20 |
| 1 | |||
| 264 | 1 1 lxbfYeaa | -1 OR 3*2>(0+5+810-810) | 2025/04/20 |
| 1 | |||
| 265 | 1 1 lxbfYeaa | -1 OR 3*2>(0+5+810-810) | 2025/04/20 |
| 1 | |||
| 266 | 1 1 lxbfYeaa | -1' OR 2+688-688-1=0+0+0+1 -- | 2025/04/20 |
| 1 | |||
| 267 | 1 1 lxbfYeaa | -1' OR 3+688-688-1=0+0+0+1 -- | 2025/04/20 |
| 1 | |||
| 268 | 1 1 lxbfYeaa | -1' OR 3*2<(0+5+688-688) -- | 2025/04/20 |
| 1 | |||
| 269 | 1 1 lxbfYeaa | -1' OR 3*2>(0+5+688-688) -- | 2025/04/20 |
| 1 | |||
| 270 | 1 1 lxbfYeaa | -1' OR 2+485-485-1=0+0+0+1 or 'tJ8OGFR0'=' | 2025/04/20 |
| 1 | |||
| 271 | 1 1 lxbfYeaa | -1' OR 3+485-485-1=0+0+0+1 or 'tJ8OGFR0'=' | 2025/04/20 |
| 1 | |||
| 272 | 1 1 lxbfYeaa | -1' OR 3*2<(0+5+485-485) or 'tJ8OGFR0'=' | 2025/04/20 |
| 1 | |||
| 273 | 1 1 lxbfYeaa | -1' OR 3*2>(0+5+485-485) or 'tJ8OGFR0'=' | 2025/04/20 |
| 1 | |||
| 274 | 1 1 lxbfYeaa | -1" OR 2+514-514-1=0+0+0+1 -- | 2025/04/20 |
| 1 | |||
| 275 | 1 1 lxbfYeaa | -1" OR 3+514-514-1=0+0+0+1 -- | 2025/04/20 |
| 1 | |||
| 276 | 1 1 lxbfYeaa | -1" OR 3*2<(0+5+514-514) -- | 2025/04/20 |
| 1 | |||
| 277 | 1 1 lxbfYeaa | -1" OR 3*2>(0+5+514-514) -- | 2025/04/20 |
| 1 | |||
| 278 | 1 1 lxbfYeaa | if(now()=sysdate(),sleep(15),0) | 2025/04/20 |
| 1 | |||
| 279 | 1 1 lxbfYeaa | Mr.0'XOR(if(now()=sysdate(),sleep(15),0))XOR'Z | 2025/04/20 |
| 1 | |||
| 280 | 1 1 lxbfYeaa | Mr.0"XOR(if(now()=sysdate(),sleep(15),0))XOR"Z | 2025/04/20 |
| 1 | |||
| 281 | 1 1 lxbfYeaa | (select(0)from(select(sleep(15)))v)/*'+(select(0)from(select(sleep(15)))v)+'"+(select(0)from(select(sleep(15)))v)+"*/ | 2025/04/20 |
| 1 | |||
| 282 | 1 1 lxbfYeaa | Mr.-1 waitfor delay '0:0:15' -- | 2025/04/20 |
| 1 | |||
| 283 | 1 1 lxbfYeaa | Mr.38u2eHvv'; waitfor delay '0:0:15' -- | 2025/04/20 |
| 1 | |||
| 284 | 1 1 lxbfYeaa | Mr.LF6AvBV7' OR 542=(SELECT 542 FROM PG_SLEEP(15))-- | 2025/04/20 |
| 1 | |||
| 285 | 1 1 lxbfYeaa | Mr.BGX4H0eE') OR 459=(SELECT 459 FROM PG_SLEEP(15))-- | 2025/04/20 |
| 1 | |||
| 286 | 1 1 lxbfYeaa | Mr.wIgLGnpl')) OR 305=(SELECT 305 FROM PG_SLEEP(15))-- | 2025/04/20 |
| 1 | |||
| 287 | 1 1 lxbfYeaa | Mr.'||DBMS_PIPE.RECEIVE_MESSAGE(CHR(98)||CHR(98)||CHR(98),15)||' | 2025/04/20 |
| 1 | |||
| 288 | 1 1 lxbfYeaa | Mr.'||DBMS_PIPE.RECEIVE_MESSAGE(CHR(98)||CHR(98)||CHR(98),15)||' | 2025/04/20 |
| 1 | |||
| 289 | 1 1 lxbfYeaa | Mr.'||DBMS_PIPE.RECEIVE_MESSAGE(CHR(98)||CHR(98)||CHR(98),6)||' | 2025/04/20 |
| 1 | |||
| 290 | 1 1 lxbfYeaa | Mr.'||DBMS_PIPE.RECEIVE_MESSAGE(CHR(98)||CHR(98)||CHR(98),3)||' | 2025/04/20 |
| 1 | |||
| 291 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||
| 292 | 1 1 lxbfYeaa | Mr.'" | 2025/04/20 |
| 1 | |||
| 293 | 1 1 lxbfYeaa | Mr.是摺%2527%2522\'\" | 2025/04/20 |
| 1 | |||
| 294 | 1 1 lxbfYeaa | @@wUYWV | 2025/04/20 |
| 1 | |||
| 295 | 1 1 lxbfYeaa | Mr. | 2025/04/20 |
| 1 | |||